Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers

Electrospinning, the only method that can continuously produce nanofibers, has been widely used to prepare nanofibers for tissue engineering applications. However, electrospinning is not suitable preparing clinically relevant three-dimensional (3D) nanofibrous scaffolds with hierarchical pore structures. In this study, recombinant human collagen (RHC)/chitosan prepared by were combined porous produced freeze drying fabricate 3D scaffolds. These exhibited high porosity (over 80%) and an interconnected structure (ranging from sub-micrometers 200 μm) covered nanofibers. As confirmed characterization results, these showed good swelling ability, stability, adequate mechanical strength, making it possible use in various addition, after seven days of cell culturing, NIH 3T3 was infiltrated into while maintaining its morphology superior proliferation viability. results indicated hold great promise